Apoptosis of renal cortical cells in the hemolytic-uremic syndrome: : In vivo and in vitro studies
Författare
Summary, in English
This study examined apoptotic cell death associated with Shiga-like toxin (Stx)-producing Escherichia coli. Renal cortices from three children with postenteropathic hemolytic-uremic syndrome (HUS) and from mice infected with E. coli O157:H7 and pediatric renal tubular epithelial cells stimulated with Stx and E. coli O157:H7 extracts were examined for apoptotic changes. Apoptotic cells were detected by terminal dUTP nick end labeling of tubuli and glomeruli from HUS patients and from mice inoculated with Stx-2-positive and Stx-negative strains. Apoptosis was more extensive and severe ultramorphological nuclear and cytoplasmic changes were seen in the Stx-2-positive group. Stx caused DNA fragmentation and ultramorphological changes indicating apoptosis in cultured pediatric tubular cells. DNA fragmentation increased when cells were pre-stimulated with tumor necrosis factor alpha. Polymyxin extracts from Stx-2-positive and Stx-negative strains induced DNA fragmentation, but only extracts from Stx-2-positive strains caused ultramorphological changes and extensive DNA fragmentation. The results indicate that HUS is accompanied by increased apoptosis of kidney cells and that bacterial factors, possibly together with host cytokines in vivo, may activate apoptotic tissue injury.
Avdelning/ar
Publiceringsår
1998-02
Språk
Engelska
Sidor
636-644
Publikation/Tidskrift/Serie
Infection and Immunity
Volym
66
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
American Society for Microbiology
Ämne
- Microbiology in the medical area
Nyckelord
- Animals
- Apoptosis
- Bacterial Toxins
- Child, Preschool
- Female
- Hemolytic-Uremic Syndrome
- Humans
- Infant
- Kidney Cortex
- Male
- Mice
- Mice, Inbred C3H
- Microscopy, Electron
- Polymyxins
- Shiga Toxin 2
- Tumor Necrosis Factor-alpha
Status
Published
Forskningsgrupp
- Pediatric Nephrology
ISBN/ISSN/Övrigt
- ISSN: 0019-9567