HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.
Författare
Summary, in English
The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.
Publiceringsår
2013
Språk
Engelska
Sidor
297-307
Publikation/Tidskrift/Serie
Methods in Molecular Biology
Volym
919
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Pharmacology and Toxicology
- Medicinal Chemistry
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1940-6029