Meny

Javascript verkar inte påslaget? - Vissa delar av Lunds universitets webbplats fungerar inte optimalt utan javascript, kontrollera din webbläsares inställningar.
Du är här

Use of synchrotron-radiation-based FTIR imaging for characterizing changes in cell contents

Författare:
Publiceringsår: 2012
Språk: Engelska
Sidor: 1311-1316
Publikation/Tidskrift/Serie: Analytical and Bioanalytical Chemistry
Volym: 404
Nummer: 5
Dokumenttyp: Artikel
Förlag: Springer

Sammanfattning

FTIR imaging of individual cells is still limited by the low signal-to-noise ratio obtained from analysis of such weakly absorbing organic matter when using a Globar IR source. In this study, we used FTIR imaging with a synchrotron radiation source and a focal plane array detector to determine changes in the cellular contents of cryofixed cells after culture for 48 h on Si3N4 substrate. Several spectral differences were observed for cells deprived of glucose compared with control cells: a lower amide I-to-amide II ratio (P < 0.01); a different secondary structure profile of proteins (obtained from amide I spectral region curve fitting), with a significant increase in non-ordered structure components (P < 0.01); and a higher nu(C = C-H)/nu (as)(CH3) absorption ratio (P < 0.01), suggesting increased unsaturation of fatty acyl chains. Therefore, our study has shown that FTIR imaging with a synchrotron radiation source enables determination of several spectral changes of individual cells between two experimental conditions, which thus opens the way to cell biology studies with this vibrational spectroscopy technique.

Disputation

Nyckelord

  • Chemistry
  • Bioanalytical methods
  • Spectroscopy/instrumentation
  • IR
  • spectroscopy/Raman spectroscopy
  • Imaging
  • Synchrotron radiation

Övriga

Published
Yes
  • ISSN: 1618-2642

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen