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Immobilisation of DNA to polymerised SU-8 photoresist

Författare:
  • Rodolphe Marie
  • Silvan Schmid
  • Alicia Johansson
  • Louise Ejsing
  • Maria Nordström (Dr)
  • Daniel Häfliger
  • Claus BV Christensen
  • Anja Boisen
  • Martin Dufva
Publiceringsår: 2006
Språk: Engelska
Sidor: 1327-1332
Publikation/Tidskrift/Serie: Biosensors and Bioelectronics
Volym: 21
Nummer: 7
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems.

Disputation

Nyckelord

  • Technology and Engineering

Övriga

Published
Yes
  • ISSN: 0956-5663

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