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In vitro molecular evolution of antibody genes mimicking receptor revision

Författare:
Publiceringsår: 2002
Språk: Engelska
Sidor: 349-356
Publikation/Tidskrift/Serie: MOLECULAR IMMUNOLOGY
Volym: 39
Nummer: 5-6
Dokumenttyp: Artikel
Förlag: PERGAMON-ELSEVIER SCIENCE LTD

Sammanfattning

Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • Technology and Engineering
  • phage display
  • affinity maturation
  • antibody
  • receptor revision
  • somatic hypermutation

Övriga

Published
Yes
  • ISSN: 0161-5890

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