Plasma enterostatin: Identification and release in rats in response to a meal
Författare
Summary, in English
Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with I-125-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma. Discussion: The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake.
Avdelning/ar
Publiceringsår
2002
Språk
Engelska
Sidor
688-694
Publikation/Tidskrift/Serie
Obesity Research
Volym
10
Issue
7
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Nature Publishing Group
Ämne
- Other Clinical Medicine
- Nutrition and Dietetics
Nyckelord
- fat intake
- procolipase
- appetite
- sucrose
- enzyme-linked immunosorbent
- assay
Status
Published
Forskningsgrupp
- Appetite Regulation
ISBN/ISSN/Övrigt
- ISSN: 1071-7323