Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Tenascins are associated with lipid rafts isolated from mouse brain

In English

Författare

  • J Kappler
  • SL Baader
  • S Franken
  • P Pesheva
  • K Schilling
  • Uwe Rauch
  • V Gieselmann

Summary, in English

Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts. (C) 2002 Elsevier Science (USA). All rights reserved.

Avdelning/ar

Publiceringsår

2002

Språk

Engelska

Sidor

742-747

Publikation/Tidskrift/Serie

Biochemical and Biophysical Research Communications

Volym

294

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Biological Sciences

Nyckelord

  • cerebellum
  • tenascin
  • lipid rafts

Status

Published

Forskningsgrupp

  • Vessel Wall Biology

ISBN/ISSN/Övrigt

  • ISSN: 1090-2104