The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
Författare
Summary, in English
In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44- and CD24- were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44+ and CD24- were absent in a significant number of women with a family history of breast cancer. The CD44+/CD24- immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women.
Avdelning/ar
- EpiHealth: Epidemiology for Health
- Tumörmikromiljö
- Institutionen för kliniska vetenskaper, Lund
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Medicinsk onkologi
Publiceringsår
2016-05-27
Språk
Engelska
Sidor
117-124
Publikation/Tidskrift/Serie
Breast Cancer: Targets and Therapy
Volym
8
Dokumenttyp
Artikel i tidskrift
Förlag
Dove Medical Press Ltd.
Ämne
- Cancer and Oncology
Nyckelord
- BRCA1 gene
- Human
- Immunohistochemistry
- Mammary glands
- Neoplasms
- Stem cells
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1179-1314