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A New Easy-to-Prepare Homogeneous Continuous Electrochromatographic Bed for Enantiomer Recognition

In English

Författare

  • Ákos Végvári
  • András Földesi
  • Csaba Hetényi
  • Olga Kochegarova
  • Martin G. Schmid
  • Vilma Kudirkaite
  • Stellan Hjertén

Summary, in English

Completely homogeneous polyacrylamide-based gels were used for capillary electrochromatography (CEC) of drug enantiomers. Like continuous beds (also called continuous polymer rods, silica rods, monoliths) they do not require frits to support the bed, because it is covalently linked to the capillary wall. Along lifetime is an important feature of the beds. The gel matrices can be prepared in any laboratory and for specific interactions they can be derivatized with appropriate ligands. The application range is, therefore, broad. For chiral electrochromatography, negatively and positively charged polyacrylamide gels copolymerized with 2-hydroxy-3-allyloxy-propyl-b-cyclodextrin (allyl-b-CD) were prepared. The latter monomer was synthesized from b-CD and allyl-glycidyl ether by a very simple one-step procedure. Eight acidic, neutral and basic drug compounds were resolved into their enantiomers, most of them with baseline separation. Interestingly, the resolution is independent of the electroendosmotic velocity, i.e., rapid analyses will not give low resolution. Upon increasing this velocity, the plate height for the fast enantiomer did not change (or decreased slightly), whereas that for the slow enantiomer increased. Only the last term in the van Deemter equation contributed significantly to the total plate height. The composition of the gel was chosen such that the "pores" became large enough to guarantee a satisfactory electroendosmotic flow (EOF). This open gel structure explains why acetone diffused as in free solution, i.e., independently of the presence of the gel matrix. This finding also indicates that the separation of small molecules in polyacrylamide gels cannot be explained by "molecular-sieving", but rather by some type of adsorption ("aromatic adsorption"?).

Publiceringsår

2000

Språk

Engelska

Sidor

3116-3125

Publikation/Tidskrift/Serie

Electrophoresis

Volym

21

Issue

15

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Medical Engineering

Nyckelord

  • Enantiomer separation
  • Continuous beds
  • Homogeneous gels
  • Electrochromatography
  • Allylated-b-cyclodextrin
  • van Deemter equation

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0173-0835