Chiral Separation of Amino Acids by Ligand-Exchange Electrochromatography Using Continuous Beds

Summary, in English

A chiral ligand-exchange phase for capillary electrochromatography based on continu-ous bed technology was developed. The chiral stationary phase is prepared by a one- step in situ copolymerization procedure using methacrylamide, piperazine diacryla- mide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chi- ral separation of underivatized amino acids is demonstrated.

• Författare: Martin G. Schmid; Nina Grobuschek; Claudia Tuscher; Gerald Gübitz; Ákos Végvári; Egidijus Machtejevas; Audrius Maruska; Stellan Hjertén
• Publiceringsår: 2000
• Språk: Engelska
• Sidor: 3141-3144
• Publikation/Tidskrift/Serie: Electrophoresis
• Volym: 21
• Issue: 15
• Länkar: http://dx.doi.org/10.1002/1522-2683(20000901)21:15<3141::AID-ELPS3141>3.0.CO;2-T
• Dokumenttyp: Artikel i tidskrift
• Förlag: John Wiley & Sons Inc.
• Ämne: Medical Engineering
• Nyckelord: Capillary electrochromatography; Enantiomer separation; Ligand-exchange; Continuous beds; Chiral beds; Monolithic phases
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 0173-0835