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Design of recombinant antibody microarrays for cell surface membrane proteomics

In English

Författare

  • Linda Dexlin Mellby
  • Johan Ingvarsson
  • Björn Frendeus
  • Carl Borrebaeck
  • Christer Wingren

Summary, in English

Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.

Publiceringsår

2008

Språk

Engelska

Sidor

319-327

Publikation/Tidskrift/Serie

Journal of Proteome Research

Volym

7

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1535-3893