Mutations in HPV16 L1 hypervariable surface-exposed loops affect L2 binding and DNA encapsidation.
Författare
Summary, in English
Prophylactic vaccines against human papillomavirus (HPV) based on Virus-Like Particles (VLPs) induce type-specific neutralizing antibodies against a small number of hypervariable residues positioned in surface-exposed loops of the major capsid protein L1. To investigate the importance of these residues for neutralization, cross-neutralization, L2 incorporation and genome encapsidation, ten surface-exposed amino acid residues in 4 hypervariable loops of L1 were mutated. VLPs containing mutated or wild type L1, with or without wild type L2, were produced in 293TT cells using pseudovirion expression vectors. The mutations reduced the ability to induce neutralizing antibodies and to incorporate the L2 protein in the capsid. Ability to induce cross-neutralizing antibodies and to encapsidate pseudogenomes were completely abrogated. In summary, the surface-exposed L1 loops are important for the function of the HPV particle.
Avdelning/ar
- Klinisk mikrobiologi, Malmö
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publiceringsår
2013
Språk
Engelska
Sidor
1841-1849
Publikation/Tidskrift/Serie
Journal of General Virology
Volym
94
Issue
May,8
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Microbiology Society
Ämne
- Microbiology in the medical area
Status
Published
Forskningsgrupp
- Clinical Microbiology, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1465-2099