Fetal hepatic progenitors support long-term expansion of hematopoietic stem cells

Summary, in English

We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK- cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver. (C) 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

• Författare: Song Chou; Johan Flygare; Harvey F. Lodish
• Avdelning/ar: Avdelningen för molekylärmedicin och genterapi; StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
• Publiceringsår: 2013
• Språk: Engelska
• Sidor: 479-490
• Publikation/Tidskrift/Serie: Experimental Hematology
• Volym: 41
• Issue: 5
• Länkar: http://dx.doi.org/10.1016/j.exphem.2013.02.003
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Hematology
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1873-2399