Frequency determination of rare populations by flow cytometry: A hematopoietic stem cell perspective.
Författare
Summary, in English
Flow cytometry allows for identification of cellular subsets based on cell intrinsic properties, most often by the use of fluorochrome-conjugated antibodies recognizing distinct cell-surface epitopes that define the cells of interest. Advances in technical instrumentation and the availability of an ever-increasing number of fluorophores, today enables identification of multicolor defined cellular populations to a previously unreachable resolution. However, these possibilities put an increasing demand on preparation, acquisition, and subsequent analysis of the investigated samples. Identification of very rare cellular subsets, such as the bone marrow-residing hematopoietic stem cells (HSCs), causes further complexity to such analysis. Here, we discuss considerations and aspects in multicolor flow cytometry as exemplified by analysis of human and mouse HSCs. We illustrate advantages and drawbacks of polychromatic flow cytometry and propose strategies, such as the use of internal reference populations, for sample analysis. © 2013 International Society for Advancement of Cytometry.
Avdelning/ar
- Hematopoietic and immunologic developement
- Institutionen för experimentell medicinsk vetenskap
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
Publiceringsår
2013
Språk
Engelska
Sidor
721-727
Publikation/Tidskrift/Serie
Cytometry Part A
Volym
83A
Issue
8
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Cell and Molecular Biology
Status
Published
Forskningsgrupp
- Hematopoietic and immunologic developement
ISBN/ISSN/Övrigt
- ISSN: 1552-4930