Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

Summary, in English

The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.

• Författare: Åke Lundwall; U. Hellman; G. Eggertsen; J. Sjöquist
• Publiceringsår: 1984
• Språk: Engelska
• Sidor: 57-62
• Publikation/Tidskrift/Serie: FEBS Letters
• Volym: 169
• Issue: 1
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6714418
• Dokumenttyp: Artikel i tidskrift
• Förlag: Wiley-Blackwell
• Ämne: Medicinal Chemistry
• Nyckelord: *Cyanogen Bromide; Complement C3/*analysis; High Pressure Liquid; Gel; Chromatography; Amino Acid Sequence; Amino Acids/analysis; Humans; Peptide Fragments/*analysis; Research Support; Non-U.S. Gov't
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1873-3468