Epstein-Barr virus binding to virus-carrying cell lines is enhanced in the presence of C3 and C3d

Summary, in English

The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are distinct, although they both involve the same cell surface complex. The two receptors are believed to display cooperativity.

• Författare: A. Wells; N. Koide; G. Eggertsen; Åke Lundwall; T. Godal; H. B. Steen; G. Klein
• Publiceringsår: 1984
• Språk: Engelska
• Sidor: 13-507
• Publikation/Tidskrift/Serie: Journal of General Virology
• Volym: 65 ( Pt 3)
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6321638
• Dokumenttyp: Artikel i tidskrift
• Förlag: Microbiology Society
• Ämne: Medicinal Chemistry
• Nyckelord: Complement/*analysis; Receptors; Kinetics; Humans; Human/*metabolism; Herpesvirus 4; Complement C3d; Cell Line; Complement C3/*metabolism; Virus/*analysis; Research Support; Non-U.S. Gov't; U.S. Gov't; P.H.S.
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1465-2099