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Semenogelin, the predominant protein in human semen. Primary structure and identification of closely related proteins in the male accessory sex glands and on the spermatozoa

In English

Författare

Summary, in English

The predominant protein in human semen, semenogelin, was characterized by lambda gt11 clones isolated from a seminal vesicular cDNA library. One clone, carrying a cDNA insert of 1606 nucleotides and a polyadenylated tail, coded for the entire semenogelin precursor. An open reading frame of 1386 nucleotides encodes a signal peptide and the mature protein of 439 amino acid residues, in which residues 85-136 are identical with a previously characterized semenogelin fragment. The polypeptide chain displays a most conspicuous region of internal sequence homology where 46 of the 58 amino acid residues at positions 259-316 are repeated at positions 319-376. An abundant seminal vesicular transcript of 1.8 kilobases (kb) codes for semenogelin. Two additional transcripts, one seminal vesicular 2.2-kb species and one epididymal 2.0-kb species, code for related proteins that have a close structural relationship as well as antigenic epitopes in common with semenogelin. Semenogelin and the semenogelin-related proteins are the major proteins involved in the gelatinous entrapment of ejaculated spermatozoa. Antigenic epitopes common to these proteins are localized to the parts of the spermatozoa involved in locomotion. The spermatozoa become progressively motile as the gel-forming proteins are fragmented by the kallikrein-like protease, prostate-specific antigen, and the gel dissolves.

Avdelning/ar

Publiceringsår

1989

Språk

Engelska

Sidor

900-1894

Publikation/Tidskrift/Serie

Journal of Biological Chemistry

Volym

264

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Biochemistry and Molecular Biology

Ämne

  • Urology and Nephrology
  • Medicinal Chemistry

Nyckelord

  • Molecular Sequence Data
  • Male
  • Immunohistochemistry
  • Humans
  • Gonadal Steroid Hormones/*analysis/genetics
  • Male/*analysis
  • Genitalia
  • DNA/analysis
  • Amino Acid Sequence
  • Base Sequence
  • Research Support
  • Non-U.S. Gov't
  • Semen/*analysis
  • *Seminal Vesicle Secretory Proteins
  • Spermatozoa/*analysis

Status

Published

Forskningsgrupp

  • Clinical Chemistry, Malmö
  • Urological research, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 0021-9258