Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring

In English

Författare

Summary, in English

Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).

Publiceringsår

2013

Språk

Engelska

Sidor

4612-4616

Publikation/Tidskrift/Serie

Journal of Proteome Research

Volym

12

Issue

10

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Medical Engineering

Nyckelord

  • Chromosome 19
  • Human kallikrein-2
  • Selected reaction monitoring
  • Mass spectrometry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1535-3893