Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89

Summary, in English

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na(+)/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate.

• Författare: Palanivelu Sengottaiyan; Jitka Petrlova; Jens O Lagerstedt; Lorena Ruiz-Pavon; Madhu S. Budamagunta; John C Voss; Bengt L Persson
• Avdelning/ar: Institutionen för experimentell medicinsk vetenskap
• Publiceringsår: 2013-07-05
• Språk: Engelska
• Sidor: 551-556
• Publikation/Tidskrift/Serie: Biochemical and Biophysical Research Communications
• Volym: 436
• Issue: 3
• Länkar: http://dx.doi.org/10.1016/j.bbrc.2013.06.011
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Biological Sciences
• Nyckelord: Pho89; Pichia pastoris; Oligomer; Reconstitution; Phosphate transport; Circular dichroism
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1090-2104