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Calpain inhibitors delay injury-induced apoptosis in adult mouse spinal cord motor neurons

Författare:
  • HamidReza Momeni
  • Martin Kanje
Publiceringsår: 2006
Språk: Engelska
Sidor: 761-765
Publikation/Tidskrift/Serie: Neuroreport
Volym: 17
Nummer: 8
Dokumenttyp: Artikel
Förlag: Lippinkott Williams & Wilkins

Sammanfattning

Here, we investigated the effect of calpain inhibitors on apoptosis in organotypic adult spinal cord slices from mice. An increase in calpain I immunoreactivity was found in the nuclei of motor neurons from slices cultured for 30 min. After 4 h, the immunopositive motor neurons exhibited apoptotic changes including nuclear and chromatin condensation. Eight hours after excision, most motor neurons showed nuclear apoptotic features. Two calpain inhibitors, leupeptin and calpain inhibitor XI, inhibited apoptosis in the motor neurons while the caspase inhibitor Z-VAD.fmk had no effect. Leupeptin, but not calpain inhibitor XI and Z-VAD.fmk, also inhibited nucleosomal DNA fragmentation. These results suggest the involvement of calpain I in the induction of apoptosis in motor neurons of adult spinal cord and that apoptosis can be triggered independent of caspase activation.

Disputation

Nyckelord

  • Biology and Life Sciences
  • spinal cord
  • culture
  • organ
  • motor neurons
  • caspase
  • calpain inhibitor
  • apoptosis
  • calpain

Övriga

Published
Yes
  • ISSN: 0959-4965

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