Purification and functional characterization of factor I.
Författare
Summary, in English
Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.
Avdelning/ar
Publiceringsår
2014
Språk
Engelska
Sidor
177-188
Publikation/Tidskrift/Serie
Methods in Molecular Biology
Volym
1100
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Other Basic Medicine
Status
Published
Forskningsgrupp
- Protein Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1940-6029