Purification and Functional Characterization of C4b-Binding Protein (C4BP).
Författare
Summary, in English
C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.
Avdelning/ar
Publiceringsår
2014
Språk
Engelska
Sidor
169-176
Publikation/Tidskrift/Serie
Methods in Molecular Biology
Volym
1100
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Other Basic Medicine
Status
Published
Forskningsgrupp
- Protein Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1940-6029