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Proficiency of drug susceptibility testing of Mycobacterium tuberculosis against pyrazinamide: the Swedish experience

In English

Författare

  • S. Hoffner
  • K. Angeby
  • Erik Sturegård
  • B. Jonsson
  • A. Johansson
  • M. Sellin
  • J. Werngren

Summary, in English

BACKGROUND: Pyrazinamide (PZA) is a key drug in the treatment of tuberculosis (TB), including multidrug-resistant TB. Drug susceptibility testing (DST) of Mycobacterium tuberculosis against PZA is not included in the World Health Organization's yearly proficiency testing. There is an increasing need to establish quality control of PZA DST. OBJECTIVE: To evaluate the performance of PZA DST and to introduce a quality assurance system for the test in Sweden. METHOD: Panels with PZA-susceptible and -resistant isolates were used in three rounds of proficiency testing in all five Swedish clinical TB laboratories and our reference laboratory. All laboratories used the MGIT 960 system. Minimum inhibitory concentrations (MICs) were determined and the pncA gene was sequenced to further characterise the 52 panel strains. RESULTS: Good agreement was seen between the phenotypic PZA DST and pncA sequence data, and MIC determination confirmed high levels of resistance. However, in contrast to other drugs, for which correct proficiency test results were observed, specificity problems occurred for PZA DST in some laboratories. CONCLUSIONS: In Sweden, using panel testing, differences were seen in the proficiency of TB laboratories in correctly identifying PZA susceptibility. Improved results were noted in the third round; PZA has therefore been included in yearly proficiency testing.

Publiceringsår

2013

Språk

Engelska

Sidor

1486-1490

Publikation/Tidskrift/Serie

The International Journal of Tuberculosis and Lung Disease

Volym

17

Issue

11

Dokumenttyp

Artikel i tidskrift

Förlag

International Union against Tuberculosis and Lung Disease

Ämne

  • Respiratory Medicine and Allergy

Nyckelord

  • tuberculosis
  • pyrazinamide
  • proficiency testing
  • MDR-TB

Status

Published

Forskningsgrupp

  • Clinical Microbiology, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 1815-7920