Molecular cloning of human prostate specific antigen cDNA

Summary, in English

A lambda gt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library. The cDNA insert of 1415 nucleotides hybridizes specifically to a prostate mRNA species of 1.5 kb. The nucleotide sequence codes for part of a signal peptide, a short propiece and the mature protein of 237 amino acid residues. The Mr for the non-glycosylated protein was 26,089. One potential site for N-linked carbohydrate attachment was identified. The primary structure shows extensive homology with proteases of the kallikrein family.

• Författare: Åke Lundwall; Hans Lilja
• Avdelning/ar: Klinisk kemi, Malmö
• Publiceringsår: 1987
• Språk: Engelska
• Sidor: 22-317
• Publikation/Tidskrift/Serie: FEBS Letters
• Volym: 214
• Issue: 2
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2436946
• Dokumenttyp: Artikel i tidskrift
• Förlag: Wiley-Blackwell
• Ämne: Medicinal Chemistry
• Nyckelord: Non-U.S. Gov't; Research Support; Prostate-Specific Antigen; Prostate/immunology; Male; Humans; DNA/*genetics; Molecular; Cloning; Base Sequence; Amino Acid Sequence; Antigens/*genetics
• Status: Published
• Forskningsgrupp: Clinical Chemistry, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1873-3468