Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Plant stem cell maintenance involves direct transcriptional repression of differentiation program

In English

Författare

  • Ram Kishor Yadav
  • Mariano Perales
  • Jeremy Gruel
  • Carolyn Ohno
  • Marcus Heisler
  • Thomas Girke
  • Henrik Jönsson
  • G. Venugopala Reddy

Summary, in English

In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of stem cells that provide cells for all above ground organs. WUSCHEL, a homeodomain TF produced in cells of the niche, migrates into adjacent cells where it specifies stem cells. Through high-resolution genomic analysis, we show that WUSCHEL represses a large number of genes that are expressed in differentiating cells including a group of differentiation promoting TFs involved in leaf development. We show that WUS directly binds to the regulatory regions of differentiation promoting TFs; KANADI1, KANADI2, ASYMMETRICLEAVES2 and YABBY3 to repress their expression. Predictions from a computational model, supported by live imaging, reveal that WUS-mediated repression prevents premature differentiation of stem cell progenitors, being part of a minimal regulatory network for meristem maintenance. Our work shows that direct transcriptional repression of differentiation promoting TFs is an evolutionarily conserved logic for stem cell regulation.

Publiceringsår

2013

Språk

Engelska

Sidor

654-654

Publikation/Tidskrift/Serie

Molecular Systems Biology

Volym

9

Dokumenttyp

Artikel i tidskrift

Förlag

Nature Publishing Group

Ämne

  • Biophysics

Nyckelord

  • central zone
  • CLAVATA3
  • shoot apical meristem
  • stem cell niche
  • WUSCHEL

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1744-4292