Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia

Summary, in English

Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the NAD + -dependent short-chain dehydrogenases/reductases (SDRs) NV10127, NV10128, and NV10129 are linked to the ability of Nv to produce RR. Here we show by assaying recombinant enzymes that SDRs from both Nv and N. giraulti (Ng), the latter a species with only RS in the pheromone, epimerise RS into RR and vice versa with (4R)-5-oxo-4-decanolide as an intermediate. Nv-derived SDR orthologues generally had higher epimerisation rates, which were also influenced by NAD + availability. Semiquantitative protein analyses of the pheromone glands by tandem mass spectrometry revealed that NV10127 as well as NV10128 and/or NV10129 were more abundant in Nv compared to Ng. We conclude that the interplay of differential expression patterns and SDR epimerisation rates on the ancestral pheromone component RS accounts for the evolution of a novel pheromone phenotype in Nv.

• Författare: Joachim Ruther; Åsa K. Hagström; Birgit Brandstetter; John Hofferberth; Astrid Bruckmann; Florian Semmelmann; Michaela Fink; Helena Lowack; Sabine Laberer; Oliver Niehuis; Rainer Deutzmann; Christer Löfstedt; Reinhard Sterner
• Avdelning/ar: Funktionell zoologi; BECC: Biodiversity and Ecosystem services in a Changing Climate; Feromongruppen
• Publiceringsår: 2016-10-05
• Språk: Engelska
• Publikation/Tidskrift/Serie: Scientific Reports
• Volym: 6
• Länkar: http://dx.doi.org/10.1038/srep34697
• Dokumenttyp: Artikel i tidskrift
• Förlag: Nature Publishing Group
• Ämne: Zoology; Biochemistry and Molecular Biology
• Status: Published
• Forskningsgrupp: Pheromone Group
• ISBN/ISSN/Övrigt: ISSN: 2045-2322