Flow-Through-Mediated Surface Immobilization of Sub-Micrometre Particles in Monolithic Cryogels
Författare
Summary, in English
Particles with functional groups on their surface were immobilized on premade cryogels through covalent bonding to maximize the accessibility of the target molecules to the surface of the particles. The physical properties of the composite cryogel were characterized by SEM, texture analysis and nitrogen adsorption. Different key factors affecting immobilization were also investigated, e.g., recirculation time and the number of functional groups on the premade cryogels. It was found that the physical characteristics of the composite cryogels prepared in this way were very similar to those of the primary matrices, while their binding capacity increased in comparison with conventionally prepared composite cryogels. The binding capacities of two different types of composite cryogel were evaluated by studying the adsorption of small molecules (copper ions and Orange G) and a large molecule (thermostable His-tagged exoglucanase).
Avdelning/ar
Publiceringsår
2014
Språk
Engelska
Sidor
631-638
Publikation/Tidskrift/Serie
Macromolecular Materials and Engineering
Volym
299
Issue
5
Dokumenttyp
Artikel i tidskrift
Förlag
Wiley-Blackwell
Ämne
- Industrial Biotechnology
Nyckelord
- composite
- covalent interaction
- flow-through immobilization
- macroporous hydrogel
- post modification
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1438-7492