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Transport of a hyaluronan-binding protein in brain tissue

In English

Författare

  • Joachim Kappler
  • Oliver Hegener
  • Stephan L. Baader
  • Sebastian Franken
  • Volkmar Gieselmann
  • Hanns Haeberlein
  • Uwe Rauch

Summary, in English

Hyaluronan is an unsulfated linear glycosaminoglycan with the ability to nucleate extracellular matrices by the formation of aggregates with lecticans. These matrices are essential during development of the central nervous system. In the prospective white matter of the developing brain hyaluronan is organized into fiber-like structures according to confocal microscopy of fixed slices which may guide the migration of neural precursor cells [Baier, C., S.L Baader, J. Jankowski, V. Gieselmann, K. Schilling, U. Rauch, and J. Kappler. 2007. Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum. Matrix Biol. 26: 348-58]. By using plasmon surface resonance, microinjection into brain slices and fluorescence correlation spectroscopy, we show that the brain-specific lecticans bind to, but also dissociate rather rapidly from hyaluronan. After microinjection into native cerebellar slices a GFP-tagged hyaluronan-binding neurocan fragment was enriched at binding sites in the prospective white matter, which had a directional orientation and formed local stationary concentration gradients in areas where binding sites are abundant. Fluorescence correlation spectroscopy measurements at fixed brain slices revealed that fiber-bound neurocan-GFP was mobile with Dfiber(neurocan-GFP) = 4 x 10(-10) cm(2)/s. Therefore, we propose that hyaluronan-rich fibers in the prospective white matter of the developing mouse cerebellum can guide the diffusion of lecticans. Since lecticans bind a variety of growth and mobility factors, their guided diffusion may contribute to the transport of these polypeptides and to the formation of concentration gradients. This mechanism could serve to encode positional information during development. (C) 2009 Elsevier B.V. All rights reserved.

Avdelning/ar

Publiceringsår

2009

Språk

Engelska

Sidor

396-405

Publikation/Tidskrift/Serie

Matrix Biology

Volym

28

Issue

7

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Cell and Molecular Biology

Nyckelord

  • diffusion
  • Guided
  • Neurocan
  • Lectican
  • Extracellular matrix
  • Brain development

Status

Published

Forskningsgrupp

  • Vessel Wall Biology

ISBN/ISSN/Övrigt

  • ISSN: 1569-1802