Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis
Författare
Summary, in English
Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.
Avdelning/ar
- Institutionen för immunteknologi
- Beräkningsbiologi och biologisk fysik - Genomgår omorganisation
- Institutionen för translationell medicin
- Avdelningen för translationell cancerforskning
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publiceringsår
2011
Språk
Engelska
Sidor
1645-1656
Publikation/Tidskrift/Serie
Journal of Proteome Research
Volym
10
Issue
4
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Biophysics
- Immunology in the medical area
Nyckelord
- isotopic labeling
- HPLC-MS
- 2D-PAGE
- plasma membrane
- protein
- expression
- mRNA expression
- correlation
- hypoxia
- normoxia
- neuroblastoma
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1535-3893