Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.
Författare
Summary, in English
The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.
Avdelning/ar
Publiceringsår
2015
Språk
Engelska
Sidor
1325-1334
Publikation/Tidskrift/Serie
Chemistry and Biology
Volym
22
Issue
10
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Cell Press
Ämne
- Microbiology
- Forest Science
Status
Published
Projekt
- MICCS - Molecular Interactions Controlling soil Carbon Sequestration
Forskningsgrupp
- Microbial Ecology
ISBN/ISSN/Övrigt
- ISSN: 1879-1301