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Cyclin A1 and P450 aromatase promote metastatic homing and growth of stem-like prostate cancer cells in the bone marrow.

Författare

  • Regina Miftakhova
  • Andreas Hedblom
  • Julius Semenas
  • Brian D Robinson
  • Athanasios Simoulis
  • Johan Malm
  • Albert Rizvanov
  • David Heery
  • Nigel P Mongan
  • Norman J Maitland
  • Cinzia Allegrucci
  • Jenny L Persson

Summary, in English

Bone metastasis is a leading cause of morbidity and mortality in prostate cancer (PCa). While cancer stem-like cells have been implicated as a cell of origin for PCa metastases, the pathways which enable metastatic development at distal sites remain largely unknown. In this study, we illuminate pathways relevant to bone metastasis in this disease. We observed that cyclin A1 (CCNA1) protein expression was relatively higher in PCa metastatic lesions in lymph node, lung, and bone/bone marrow. In both primary and metastatic tissues, cyclin A1 expression was also correlated with aromatase (CYP19A1), a key enzyme that directly regulates the local balance of androgens to estrogens. Cyclin A1 overexpression in the stem-like ALDHhigh subpopulation of PC3M cells, one model of PCa, enabled bone marrow integration and metastatic growth. Further, cells obtained from bone marrow metastatic lesions displayed self-renewal capability in colony forming assays. In the bone marrow, Cyclin A1 and aromatase enhanced local bone marrow-releasing factors, including androgen receptor, estrogen and matrix metalloproteinase MMP9 and promoted hte metastatic growth of PCa cells. Moreover, ALDHhigh tumor cells expressing elevated levels of aromatase stimulated tumor/host estrogen production and acquired a growth advantage in the presence of host bone marrow cells. Overall, these findings suggest that local production of steroids and MMPs in the bone marrow may provide a suitable microenvironment for ALDHhigh PCa cells to establish metastatic growths, offering new approaches to therapeutically target bone metastases.

Avdelning/ar

Publiceringsår

2016-02-26

Språk

Engelska

Sidor

2453-2464

Publikation/Tidskrift/Serie

Cancer Research

Volym

76

Issue

8

Dokumenttyp

Artikel i tidskrift

Förlag

American Association for Cancer Research Inc.

Ämne

  • Cancer and Oncology

Status

Published

Forskningsgrupp

  • Experimental Cancer Research, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 1538-7445