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Identification of the major proteoglycans from human myometrium

Författare

Summary, in English

Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total amount of proteoglycans. Heparan sulphate proteoglycans accounted for 20% and biglycan for 16%. Less than 1% consisted of the large proteoglycan versican. Conclusions. Further investigations must be performed to provide more information of the biological role of the proteoglycans in the uterus, especially during labor, by the presence of heparan sulphate proteoglycans and the minute presence of versican which indicate that the proteoglycan composition and organization is different to that of the cervix.

Avdelning/ar

  • Matrix Biology

Publiceringsår

2001

Språk

Engelska

Sidor

1084-1090

Publikation/Tidskrift/Serie

Acta Obstetricia et Gynecologica Scandinavica

Volym

80

Issue

12

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Obstetrics, Gynecology and Reproductive Medicine

Nyckelord

  • uterus
  • heparan sulphate proteoglycan
  • decorin
  • biglycan

Status

Published

Forskningsgrupp

  • Matrix Biology

ISBN/ISSN/Övrigt

  • ISSN: 1600-0412