Refractometry of microscopic objects using digital holography

In English Digital holography has some desirable properties for refractometry of microscopic
objects since it gives phase and amplitude information of an object
in all depths of focus from one set of exposures. The refractive index of the
object can be determined by observation of the movements of the Becke lines
between different focus depths. It is also shown that one single phase image
provides an independent technique to determine sign of the relief between an
object and the surrounding medium.