Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

Summary, in English

The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.

• Författare: Per Simonsson; Istvan Ferencz; Christer Alling
• Avdelning/ar: Klinisk kemi, Malmö; Experimentell Epilepsi; Neurologi, Lund; Avdelningen för klinisk kemi och farmakologi
• Publiceringsår: 1989
• Språk: Engelska
• Sidor: 169-174
• Publikation/Tidskrift/Serie: Drug and Alcohol Dependence
• Volym: 24
• Issue: 2
• Länkar: http://dx.doi.org/10.1016/0376-8716(89)90081-1
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Substance Abuse
• Nyckelord: ethanol; phospholipase C; serotonin; phosphatidic acid; platelets
• Status: Published
• Forskningsgrupp: Clinical Chemistry, Malmö; Experimental Epilepsy Group
• ISBN/ISSN/Övrigt: ISSN: 1879-0046