Extracellular MRP8/14 is a regulator of β2 integrin-dependent neutrophil slow rolling and adhesion.

Summary, in English

Myeloid-related proteins (MRPs) 8 and 14 are cytosolic proteins secreted from myeloid cells as proinflammatory mediators. Currently, the functional role of circulating extracellular MRP8/14 is unclear. Our present study identifies extracellular MRP8/14 as an autocrine player in the leukocyte adhesion cascade. We show that E-selectin-PSGL-1 interaction during neutrophil rolling triggers Mrp8/14 secretion. Released MRP8/14 in turn activates a TLR4-mediated, Rap1-GTPase-dependent pathway of rapid β2 integrin activation in neutrophils. This extracellular activation loop reduces leukocyte rolling velocity and stimulates adhesion. Thus, we identify Mrp8/14 and TLR4 as important modulators of the leukocyte recruitment cascade during inflammation in vivo.

• Författare: Monika Pruenster; Angela R M Kurz; Kyoung-Jin Chung; Xiao Cao-Ehlker; Stephanie Bieber; Claudia F Nussbaum; Susanne Bierschenk; Tanja K Eggersmann; Ina Rohwedder; Kristina Heinig; Roland Immler; Markus Moser; Uwe Koedel; Sandra Gran; Rodger P McEver; Dietmar Vestweber; Admar Verschoor; Tomas Leanderson; Triantafyllos Chavakis; Johannes Roth; Thomas Vogl; Markus Sperandio
• Avdelning/ar: Immunology
• Publiceringsår: 2015
• Språk: Engelska
• Publikation/Tidskrift/Serie: Nature Communications
• Volym: 6
• Fulltext: - 766 kB; Download statistics
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/25892652?dopt=Abstract; http://dx.doi.org/10.1038/ncomms7915
• Dokumenttyp: Artikel i tidskrift
• Förlag: Nature Publishing Group
• Ämne: Immunology in the medical area
• Status: Published
• Forskningsgrupp: Immunology
• ISBN/ISSN/Övrigt: ISSN: 2041-1723