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A modified probing feeding strategy: control aspects

Publiceringsår: 2004
Språk: Engelska
Dokumenttyp: Konferensbidrag


The paper presents a fermentation technique, which combines the advantages of the probing feeding strategy and the temperature limited fed-batch technique. The early phase of the cultivation is run under glucose limited conditions by using the original probing technique. When the maximum oxygen transfer capacity of the reactor is reached, the temperature is decreased to lower the oxygen demand. A slight glucose excess, achieved by downwards probing pulses, guarantees that thetemperature is the only limiting factor. To achieve a good control of the dissolved oxygena mid-ranging controller manipulating the stirrer speed and the temperature is used. A model describing the temperature influence on the cellmetabolism is derived to facilitate the controller design. The feeding strategy is illustrated by an experiment.



  • Technology and Engineering



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