Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Adenovirus infection enhances in vitro adherence of Streptococcus pneumoniae

In English

Författare

Summary, in English

Viruses are thought to facilitate bacterial infections of the respiratory tract, but the mechanisms are poorly understood. The present study analyzed the effect of adenovirus on bacterial adherence to human respiratory tract epithelial cells. The human lung carcinoma cell line A549 was infected with adenovirus of types 1, 2, 3, 4, 5, and 9. At a multiplicity of infection of 75 particles per cell, cytopathic effects occurred in 75 to 100% of the cells within 48 h. The virus-infected cells were harvested at various times after infection and analyzed for the ability to bind strains of Haemophilus influenzae and Streptococcus pneumoniae. Adenovirus (types 1, 2, 3, and 5) commonly causing respiratory tract infections increased the binding of adherent S. pneumoniae strains to the cells. This effect was not seen for other adenovirus types. Adenovirus infection did not change the adherence of cells of poorly adhering strains of S. pneumoniae or H. influenzae. The increase in adherence of S. pneumoniae could be inhibited by the DNA synthesis inhibitor cytosine arabinofuranoside, which is known to block the late phase of the adenovirus infection. When electron microscopy was used, there was no evidence that virus particles bound directly to bacteria. Adherence was not affected by pretreatment of the cells with virus particles or viral proteins. This suggested that adenovirus infection upregulated receptors for S. pneumoniae. The increased attachment may be one mechanism by which viruses precondition the respiratory mucosa for bacterial infection.

Publiceringsår

1994-07

Språk

Engelska

Sidor

14-2707

Publikation/Tidskrift/Serie

Infection and Immunity

Volym

62

Issue

7

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Microbiology

Ämne

  • Infectious Medicine

Nyckelord

  • Adenoviridae
  • Adenoviridae Infections
  • Bacterial Adhesion
  • Capsid
  • Capsid Proteins
  • Cells, Cultured
  • Cytarabine
  • Epithelium
  • Humans
  • Nasopharynx
  • Pneumococcal Infections
  • Streptococcus pneumoniae
  • Virus Replication

Status

Published

Forskningsgrupp

  • Experimental Infection Medicine, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 0019-9567