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Microfluidic components for protein characterization.

In English

Författare

Summary, in English

The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line digestion (12 s) and sample preparation of proteins, interfacing to capillary liquid chromatography (100 attomol sensitivity), and in-vial chemistry on femtomol amounts of sample are presented.

Publiceringsår

2001

Språk

Engelska

Sidor

75-161

Publikation/Tidskrift/Serie

Journal of Biotechnology

Volym

82

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Medical Engineering

Nyckelord

  • Miniaturization
  • Nanotechnology
  • Proteins : chemistry
  • Proteins : isolation & purification
  • Proteome
  • Silicon
  • Spectrometry
  • Mass
  • Matrix-Assisted Laser Desorption-Ionization : instrumentation
  • Equipment Design
  • Biotechnology : instrumentation
  • Bioreactors

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1873-4863