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The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolyses of p-nitrophenyl glycosides.

In English

Författare

  • Anna S Borisova
  • Sumitha Krishnaswamyreddy
  • Dina R Ivanen
  • Kirill S Bobrov
  • Elena V Eneyskaya
  • Georgy N Rychkov
  • Mats Sandgren
  • Henrik Stålbrand
  • Michael L Sinnott
  • Anna A Kulminskaya
  • Konstantin A Shabalin
Visa allt

Summary, in English

In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters kcat, Km and product KI for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α-d-galactose, affected the reaction significantly.

Publiceringsår

2015

Språk

Engelska

Sidor

43-49

Publikation/Tidskrift/Serie

Carbohydrate Research

Volym

412

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Biological Sciences

Status

Published

Projekt

  • Galactomannan degradation by fungi and gut bacteria

ISBN/ISSN/Övrigt

  • ISSN: 1873-426X