Quest for Missing Proteins: Update 2015 on Chromosome-Centric Human Proteome Project
Författare
Summary, in English
This paper summarizes the recent activities of the Chromosome-Centric Human Proteome Project (CHPP)
consortium, which develops new technologies to identify yet-to-be annotated proteins (termed
“missing proteins”) in biological samples that lack sufficient experimental evidence at the protein level
for confident protein identification. The C-HPP also aims to identify new protein forms that may be
caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic
data integration forms the basis of the C-HPP’s activities; therefore, we have summarized some of key
approaches and their roles in the project. We present new analytical technologies that improve the
chemical space and lower detection limits coupled with bioinformatics tools and some publicly
available resources that can be used to improve data analysis or support the development of analytical
assays. Most of this paper’s contents have been compiled from posters, slides, and discussions
presented in the series of C-HPP workshops held during 2014. All data (posters, presentations) used are
available at the C-HPP Wiki (http://c-hpp.webhosting.rug.nl/) and in the supporting information.
consortium, which develops new technologies to identify yet-to-be annotated proteins (termed
“missing proteins”) in biological samples that lack sufficient experimental evidence at the protein level
for confident protein identification. The C-HPP also aims to identify new protein forms that may be
caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic
data integration forms the basis of the C-HPP’s activities; therefore, we have summarized some of key
approaches and their roles in the project. We present new analytical technologies that improve the
chemical space and lower detection limits coupled with bioinformatics tools and some publicly
available resources that can be used to improve data analysis or support the development of analytical
assays. Most of this paper’s contents have been compiled from posters, slides, and discussions
presented in the series of C-HPP workshops held during 2014. All data (posters, presentations) used are
available at the C-HPP Wiki (http://c-hpp.webhosting.rug.nl/) and in the supporting information.
Publiceringsår
2015
Språk
Engelska
Sidor
3415-3431
Publikation/Tidskrift/Serie
Journal of Proteome Research
Volym
14
Issue
9
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Bioinformatics and Systems Biology
Nyckelord
- missing proteins
- chromosome centric human proteome project
- LC-MS
- antibody enrichment
- proteomics
- bioinformatics
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1535-3893