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Beta-microseminoprotein in serum correlates with the levels in seminal plasma of young, healthy males

In English

Författare

Summary, in English

Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P <.001). Significant correlations were also found in seminal plasma between MSP and prostate-specific antigen (PSA) (r =.65, P <.001) and between MSP and Zn2+ (r =.54, P <.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the prostate secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of prostate secretion, despite the contribution from extra prostatic tissues.

Publiceringsår

2008

Språk

Engelska

Sidor

330-337

Publikation/Tidskrift/Serie

Journal of Andrology

Volym

29

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

American Society of Andrology

Ämne

  • Obstetrics, Gynecology and Reproductive Medicine
  • Medicinal Chemistry

Nyckelord

  • immunoassay
  • expression
  • prostate
  • semen
  • purification

Status

Published

Forskningsgrupp

  • Clinical Chemistry, Malmö
  • Reproductive medicine, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 0196-3635