Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements
Författare
Summary, in English
This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.
Avdelning/ar
Publiceringsår
2004
Språk
Engelska
Sidor
302-311
Publikation/Tidskrift/Serie
Methods
Volym
33
Issue
4
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Endocrinology and Diabetes
Status
Published
Forskningsgrupp
- Islet cell physiology
- Diabetes - Islet Cell Exocytosis
- Neurophysiology
- Diabetes - Islet Patophysiology
ISBN/ISSN/Övrigt
- ISSN: 1095-9130