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Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo

In English

Författare

  • Aras Kadioglu
  • Jason A. Sharpe
  • Irene Lazou
  • Catharina Svanborg
  • Colin Ockleford
  • Tim J. Mitchell
  • Peter W. Andrew

Summary, in English

The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.

Publiceringsår

2001

Språk

Engelska

Sidor

105-110

Publikation/Tidskrift/Serie

FEMS Microbiology Letters

Volym

194

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Oxford University Press

Ämne

  • Microbiology in the medical area

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1574-6968