Conserved function of the Krüppel gap gene in the blowfly Lucilia sericata, despite anterior shift of expression.
Författare
Summary, in English
To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an anterior shift in expression, Kr function has remained conserved during the evolution of the blowflies.
Avdelning/ar
- Invertebrate Developmental Biology, Stefan Baumgartner's group
Publiceringsår
2011
Språk
Engelska
Sidor
257-265
Publikation/Tidskrift/Serie
Insect Molecular Biology
Volym
20
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Wiley-Blackwell
Ämne
- Developmental Biology
Status
Published
Projekt
- Formation of the bicoid gradient in Drosophila
Forskningsgrupp
- Invertebrate Developmental Biology, Stefan Baumgartner's group
ISBN/ISSN/Övrigt
- ISSN: 1365-2583