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Enhanced Ribosome and tRNA Contents in Escherichia coli Expressing a Truncated Vitreoscilla Hemoglobin Mutant Analyzed by Flow Field-Flow Fractionation

Författare

Summary, in English

The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the mass fraction of active 70S ribosomes.

Publiceringsår

2003

Språk

Engelska

Sidor

1499-1504

Publikation/Tidskrift/Serie

Biotechnology Letters

Volym

25

Issue

18

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Biochemistry and Molecular Biology
  • Analytical Chemistry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1573-6776