Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Delineating the specificity of an IgE-encoding transcriptome.

Författare

Summary, in English

Background



Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities.

Objective



We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy.

Methods



For this purpose, a human IgE library was used, constructed from peripheral blood B cells of an individual with timothy allergy. The library was screened by using phage display against a panel of 6 timothy allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, and Phl p 11).

Results



Highly diverse antibody fragments with respect to gene usage were identified. The binders were specific for their respective target antigen, except for clones selected on Phl p 6 that also recognized Phl p 5, most likely reflecting the high sequence homology between these allergens. Interestingly, by using this approach, we were able to determine the specificity of more than 25% of all IgE-producing transcripts in this individual with allergy.

Conclusion



The human IgE repertoire is produced by a limited number of highly related B-cell clones and as such is restricted in its recognition of a limited number of antigens.

Clinical implications



Human allergen-specific antibodies can, by defining the specificity of IgE responses, aid in the development of allergy vaccines or even by themselves be used in passive immunotherapy.

Publiceringsår

2007

Språk

Engelska

Sidor

1186-1192

Publikation/Tidskrift/Serie

Journal of Allergy and Clinical Immunology

Volym

120

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Respiratory Medicine and Allergy

Nyckelord

  • antibody library
  • Allergy
  • antibody repertoire
  • diversity
  • grass pollen allergen
  • IgE
  • specificity
  • phage display

Status

Published

Projekt

  • Human IgE repertoires and an anti-allergome resource

ISBN/ISSN/Övrigt

  • ISSN: 1097-6825