Ileal pelvic pouch microbiota from two former ulcerative colitis patients, analysed by DNA-based methods, were unstable over time and showed the presence of Clostridium perfringens
Författare
Summary, in English
Objective. Ileal pouch anal anastomosis ( IPAA) is the preferred method for restorative surgery in patients with ulcerative colitis who have to undergo proctocolectomy. The most common complication is pouchitis and several studies have pointed to the microbiota of the pouch as being a risk factor. The aim of this study was to follow the development of the bacterial microbiota in pouches during the first year. Material and methods. Terminal restriction fragment length polymorphism ( T-RFLP) combined with cloning and sequencing was used to identify the most predominant bacteria on the different sampling occasions. A total of 274 clones were grouped by T-RFLP and clones from each group were selected for sequencing and identified by comparison with known sequences. Results. Differences in T-RFLP profiles and clone libraries were found between the patients, and also in changes apparent in each patient at different time-points. The main bacterial groups in the pouches resembled those of the normal colonic microbiota, with a predominance of the clostridia clusters XIVa and IV, Bacteroides and Enterobacteriaceae. Exceptions were clones with sequences resembling those of the Clostridium perfringens group, in both patients and on all sampling occasions, and the dominance of clones resembling Turicibacter in one of the patients at the time of pouch construction. Conclusions. The pouch microbiota showed similarities to the normal colon microbiota except for the presence of clones with sequences resembling those of the C. perfringens group and Turicibacter. The bacterial composition differed between the two patients and the microbiota changed with time, suggesting that the composition is not stable during the first year.
Avdelning/ar
Publiceringsår
2007
Språk
Engelska
Sidor
973-985
Publikation/Tidskrift/Serie
Scandinavian Journal of Gastroenterology
Volym
42
Issue
8
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Taylor & Francis
Ämne
- Gastroenterology and Hepatology
Nyckelord
- Turicibacter
- ileal pouches
- terminal restriction fragment
- ulcerative colitis
- intestinal microbiota
- length polymorphism
Status
Published
Forskningsgrupp
- Surgery
ISBN/ISSN/Övrigt
- ISSN: 1502-7708