Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum

In English

Författare

  • Claudia Baier
  • Stephan L. Baader
  • Jakob Jankowski
  • Volkmar Gieselmann
  • Karl Schilling
  • Uwe Rauch
  • Joachim Kappler

Summary, in English

Hyaluronan is a free glycosaminoglycan which is abundant in the extracellular matrix of the developing brain. Although not covalently linked to any protein it can act as a backbone molecule forming aggregates with chondroitin sulfate proteoglycans of the lectican family and link proteins. Using neurocan-GFP as a direct histochemical probe we analyzed the distribution and organization of hyaluronan in the developing mouse cerebellum, and related its fine structure to cell types of specified developmental stages. We observed a high affinity of this probe to fiber-like structures in the prospective white matter which are preferentially oriented parallel to the cerebellar cortex during postnatal development suggesting a specially organized form of hyaluronan. In other layers of the cerebellar cortex, the hyaluronan organization seemed to be more diffuse. During the second postnatal week, the overall staining intensity of hyaluronan in the white matter declined but fiber-like structures were still present at the adult stage. This type of hyaluronan organization is different from perineuronal nets e.g. found in deep cerebellar nuclei. Double staining experiments with cell type specific markers indicated that these fiber-like structures are predominantly situated in regions where motile cells such as Pax2-positive inhibitory interneuron precursors and MBP-positive oligodendroglial cells are located. In contrast, more stationary cells such as mature granule cells and Purkinje cells are associated with lower levels of hyaluronan in their environment. Thus, hyaluronan-rich fibers are concentrated at sites where specific neural precursor cell types migrate, and the anisotropic orientation of these fibers suggests that they may support guided neural migration during brain development. (c) 2007 Elsevier B.V./International Society of Matrix Biology. All rights reserved.

Avdelning/ar

Publiceringsår

2007

Språk

Engelska

Sidor

348-358

Publikation/Tidskrift/Serie

Matrix Biology

Volym

26

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Cell and Molecular Biology

Nyckelord

  • nets
  • perineuronal
  • lectican
  • development
  • extracellular matrix
  • cerebellum

Status

Published

Forskningsgrupp

  • Vessel Wall Biology

ISBN/ISSN/Övrigt

  • ISSN: 1569-1802