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Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex

Författare:
  • Veronika Fekete
  • Maria Cierna
  • Silvia Poláková
  • Jure Piskur (Professor)
  • Pavol Sulo
Publiceringsår: 2007
Språk: Engelska
Sidor: 1237-1247
Publikation/Tidskrift/Serie: FEMS Yeast Research
Volym: 7
Nummer: 8
Dokumenttyp: Artikel
Förlag: Blackwell Publishing

Sammanfattning

Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.

Disputation

Nyckelord

  • Biology and Life Sciences
  • ethidium
  • Saccharomyces/Kluyveromyces complex
  • petite mutation
  • mtDNA
  • bromide
  • mitochondria

Övriga

Published
Yes
  • ISSN: 1567-1356

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