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High-throughput proteomics using antibody microarrays: an update

Publiceringsår: 2007
Språk: Engelska
Sidor: 673-686
Publikation/Tidskrift/Serie: Expert Review of Molecular Diagnostics
Volym: 7
Nummer: 5
Dokumenttyp: Artikel
Förlag: Future Drugs Ltd

Sammanfattning

Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will provide unique opportunities for fields such as disease diagnostics, biomarker discovery, patient stratification, predicting disease recurrence and drug target discovery. This review describes an update of high-throughput proteomics, using antibody-based microarrays, focusing on key technological advances and novel applications that have emerged over the last 3 years.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • recombinant
  • proteomics
  • protein array
  • oncoproteomics
  • nanoarrays
  • disease proteomics
  • affinity proteomics
  • antibody microarrays
  • antibody microarray

Övriga

Published
Yes
  • ISSN: 1473-7159

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